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Transient contribution of Trp86, isolated by subtracting the transient signals of WT bR and W86F. (A) Results obtained from single-wavelength measurements at 305, 307.5, 310, 312.5, and 315 nm. Each trace is shifted vertically for clarity. (B) Average of the 5 traces displayed in A, and its fit to the sum of an exponential decay (with time constant τ = 500 ± 50 fs) and a step-like function, convoluted with a Gaussian function modeling the instrument response function (IRF).
