Fig. 5.

ISG56 disrupts the interaction of MITA with VISA or TBK1. (A) ISG56 disrupts the association of MITA with VISA or TBK1 in a dose-dependent manner. 293 cells (2 × 10⁶) were transfected with the indicated plasmids (5 μg each) and an increase amount of ISG56 expression plasmids. Coimmunoprecipitation and immunoblot analysis were performed as in Fig. 1A. (B) ISG56 does not disrupt MDA5–VISA or TRIF–TBK1 interactions. 293 cells were transfected with the indicated plasmids and the coimmunoprecipitation and immunoblot analysis were performed as in Fig. 1A. (C) Kinetics of MITA–VISA and MITA–TBK1 associations after viral infection. 293 cells were left uninfected or infected with SeV for the indicated times. Immunoprecipitation and immunoblot analysis were performed with the indicated antibodies.