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Probing the E111N/K147N αHL pore for single-nucleotide discrimination in a heteropolymeric oligonucleotide. Histogram (Top) of residual current levels for E111N/K147N pores interrogated with 4 heteropolymeric DNA strands (Middle) that differ at only 1 position (blue). Gaussian fits were performed for each peak, and the mean value of the residual current for each oligonucleotide (and the standard deviation) is displayed (Bottom).
