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. 2009 Apr 24;106(19):7810–7815. doi: 10.1073/pnas.0902910106

Fig. 7.

Fig. 7.

EMSA shows that the single-stranded 5′ tail of ssiA DNA is essential for binding to RepB′. Shifted bands indicating protein/DNA complexes marked by arrows. (A) Lanes 1–4, ssiA DNA with addition of 5–12.5 μM RepB′; lane 5, only ssiA DNA. (B) The same as A with RepB′ replaced by the catalytic domain; lane 5 only ssiA DNA. (C) Lane 1, ssiA(3′Δ13) with addition of 12.5 μM RepB′; lane 2 only ssiA(3′Δ13). (D) Lane 1, ssiA(3′Δ13) with addition of 12.5 μM catalytic domain; lane 2, only ssiA(3′Δ13). (E) Lane 1, ssiA DNA without single-stranded 5′ tail [ssiA(5′Δ5)] with the addition of 12.5 μM RepB′; lane 2, only ssiA(5′Δ5). (F) Lane 1, ssiA(5′Δ5) with the addition of 12.5 μM catalytic domain; lane 2, only ssiA(5′Δ5).