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Ratio IFRET/IDirect as a function of concentration of 1a monomer in the liposome (at 70 °C). IFRET represents the emission intensity of the 560 nm PDA peak following FRET from dansyl to PDA (λex = 337 nm), and IDirect represents the emission intensity of the 560 nm peak following direct excitation (λex = 470 nm). The total concentration of 1a and 2 is 1 mM. Note that IFRET/IDirect is ~0.6 for [1a] = 1 μM but increases drastically for higher concentrations of 1a in the liposomes.
