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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Free Radic Biol Med. 2009 Feb 12;46(8):1004–1013. doi: 10.1016/j.freeradbiomed.2009.02.003

Figure 2.

Figure 2

Equations illustrating the use of the spin-trap α-phenyl-tert-butylnitrone (PBN) to trap a free radical and the subsequent metabolic reduction of the spin-adduct to a species that is not observable by electron paramagnetic resonance. Salicylate trapping of hydroxyl free radicals yields the 2,3 and 2,5-dihydroxy benzoic acid compounds noted as 2,3 and 2,5 –DHBA.