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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Apr 1;46(6):1027–1036. doi: 10.1016/j.yjmcc.2009.03.015

Figure 1. Mitochondrial localization of fura-2 in permeabilized myocytes.

Figure 1

Confocal images of intact rat ventricular cardiomyocyte simultaneously loaded with fura-2 and Mitotracker Orange before (A) and after sarcolemmal permeabilization by saponin (B). Top panels (a) are fura-2 signals, middle panels (b) are Mitotracker Orange, showing typical mitochondrial pattern and lower panels (c) show merged images. Fura-2 only colocalizes with mitochondria after permeabilization. Scale bars are equal 10 μm.