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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Exp Eye Res. 2008 Oct 4;88(2):293–306. doi: 10.1016/j.exer.2008.08.023

Figure 2.

Figure 2

FGF- and vitreous-induced phosphorylation of Akt and ERK1/2 during lens fibre differentiation. Immunofluorescent labelling of β-crystallin (D, E, F) or γ-crystallin (G, H, I) in lens explants cultured without growth factors (A, D, G), with 100ng/ml FGF (B, E, H) or with 50% vitreous (C, F, I). Cell nuclei were counterstained with Hoechst dye (A, B, C). Cells in control explants remain cuboidal in shape and do not express β– and γ-crystallin (D, G). FGF- and vitreous-treated cells elongate and accumulate β– (E, F) and γ-crystallin (H, I). Vitreous-induced fibre differentiation results in the presence of larger cells (I, arrow). Scale bar, 50 μm.