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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Exp Eye Res. 2008 Oct 4;88(2):293–306. doi: 10.1016/j.exer.2008.08.023

Figure 6.

Figure 6

Low dose of FGF-induced lens fibre differentiation, in the presence of Na3VO4. (i) Representative western blots of explants exposed to no growth factors (lane 1), 5 ng/ml of FGF for 20 min (lane 2), or FGF in the presence of 12.5μM Na3VO4 (from 20 min to 24 h; lanes 3 to 8), assayed for phosphorylated Akt (upper panel), phosphorylated ERK1/2 (middle panel) and total ERK1/2 (lower panel). (ii) Representative micrographs of cells in lens explants not exposed to growth factor (A, B, D, E) or exposed to 5 ng/ml of FGF (C, F), in the absence (A, D) or presence (B, C, E, F) of 12.5 μM Na3VO4, immunofluorescently labelled for β-crystallin (D–F) or counterstained with Hoechst dye (A–C). All representative data was taken from experiments undertaken at least three times. Scale bar, 50μm.