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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Exp Eye Res. 2008 Oct 4;88(2):293–306. doi: 10.1016/j.exer.2008.08.023

Figure 7.

Figure 7

Pulse treatment of lens explants with FGF. (i) Representative western blots of lens explants cultured continuously with 100 ng/ml FGF (lane 1), without growth factor (lane 2) and with a pulse treatment of 100 ng/ml FGF for 30 min, collected after 2 h to 18 h (lanes 3–6), assayed for phosphorylated ERK1/2 (upper panel) and total ERK1/2 (lower panel). Pulse treatment of FGF for 30 min is sufficient to activate sustained ERK1/2 phosphorylation for 12 h. (ii) Representative micrographs of cells in lens explants cultured with no growth factor (A, C), with 100 ng/ml FGF for 5 days (B, D), with a pulse-treatment of 100 ng/ml FGF for 30 min (E, G) or for 24 h (F, H), assayed for β-crystallin (C, D, G, H) or counterstained with Hoechst dye (A, B, E, F) after 5 days culture. For pulse treatment of FGF, the cells were cultured with FGF for 30 min or 24 h, then washed with M199 and further cultured in M199 without growth factor for 5 days. These cells did not elongate and express β-crystallin (G and H), similar to control explants (C). All representative data was taken from experiments undertaken at least three times. Scale bar, 50μm.