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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Int J Oncol. 2009 Jun;34(6):1613–1620. doi: 10.3892/ijo_00000291

Figure 1.

Figure 1

Expression, sub-cellular distribution and DNA binding of Forkhead transcription factor ‘O’ in normal human prostate epithelial NHPE cells and in various human prostate cancer cell lines. (A) FOXO1A, FOXO3A and FOXO4, total Akt, p-Akt (Ser473) and 14-3-3 expression in NHPE cells and in human prostate cancer 22Rv1, LNCaP, DU145 and PC-3 cells as analyzed by Western blotting. Assay with glycogen synthase kinase (GSK)-3α/β was performed to check the viability of these cells. (B) Nuclear FOXO3A DNA binding in NHPE and in various human prostate cancer cell lines. The results of DNA binding are mean ± SD of 3-4 determinations **P<0.001, compared to competitor (C) Sub-cellular distribution of FOXO3A, p-FOXO3A (Ser253), 14-3-3 and p-Akt in NHPE, LNCaP, PC-3 and DU145 cells. Anti-H4 antibody was used as loading control. (D) Binding of FOXO3A with 14-3-3. The details are described in “Materials and Methods” section. To ensure equal protein loading, the membrane was stripped and reprobed with anti-β-actin antibody.