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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Int J Oncol. 2009 Jun;34(6):1613–1620. doi: 10.3892/ijo_00000291

Figure 2.

Figure 2

AKT-dependent deregulation of FOXO3A in human prostate cancer PC-3 and DU145 cells. (A) PC-3 cells were treated with 20μM LY294002 or transfected with DN-Akt pUSEamp and control pUSEamp for 24 h. Cytosolic and nuclear fractions were isolated and subjected to Western blotting. For protein loading the blots were stripped and reprobed with β-actin antibody. (B) DU145 cells transfected with either pLNCX vector containing Akt expression plasmid or empty vector. Cytosolic and nuclear fractions were isolated and subjected to Western blotting. The details are described in “Materials and Methods” section.