Table 5.
PCR sequencing primers for pyrosequencing CYP2C9 alleles
| PCR primers | bp sizea |
2C9 allele |
Sequencing primer |
|---|---|---|---|
| Exon 3 | |||
| F: B-5′-AAACAGAGACTTACAG AGCTC-3′ |
381 | *2 | 5′-GGGCTTCCT CTTGAAC-3′ |
| R: 5′-CTAACAACCAGACTCAT AATG-3′ |
|||
| Exon 5 | |||
| F: B-5′-CAGAGCTTGGTATATGGT ATG-3′ |
323 | *6, *10 | 5′-AAGCTTTTGT TTACATTTT-3′ |
| R: 5′-TCGTAAACACAGAACTAGT CAAC-3′ |
|||
| Exon 7 | |||
| F: B-5′-CTGAATTGCTACAACAAA TGTG-3′ |
314 | *11 | 5′-TTGCATGC AGGGGCT-3′ |
| R: 5′-GATACTATGAATTTGGGAC TTC-3′ |
155 | *3, *5 | 5′-GCTGGTGG GGAGAAG-3′ |
| F: B-5′-TGCACGAGGTCCAGA GAT-3′ |
|||
| R: 5′-GATACTATGAATTTGGG ACTTC-3′ |
B, biotin-labeled primer; F, forward primer; PCR, polymerase chain reaction; R, reverse primer.
a
bp indicates PCR fragment base pair size. 2C9 allele: cytochrome 02C9 single-nucleotide polymorphism.