Skip to main content
. Author manuscript; available in PMC: 2009 May 18.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5490–5500. doi: 10.4049/jimmunol.181.8.5490

FIGURE 2.

FIGURE 2

Flow chart for determining T cell responses to SARS-CoV. Freshly isolated PBMC were stimulated with SARS-specific peptides spanning across the entire SARS-CoV proteome (1,843 overlapping peptides), and the immediate IFN-γ release by T cell responses were measured by IFN-γ ELISPOT assay as described in the Materials and Methods. The 2-dimensional matrices were set up with a total of 88 pools (1-dimensional = 43 pools; 2-dimensional = 44 pools; up to 45 peptides/pool) so that each peptide was present in two different pools. Peptides were used at a final concentration of 2 μM each.