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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Dev Biol. 2009 Mar 6;329(2):269–279. doi: 10.1016/j.ydbio.2009.02.033

Figure 4. Rasip1 ablation in MS1 cells by transient siRNA transfection hinders endothelial tube formation and migration ability.

Figure 4

A) Semi-quantitative RT-PCR (30 cycles) shows the knockdown of Rasip1 at the mRNA level. HPRT control knockdown is also shown. B,D-F) siRasip1 treated MS1 cells fail to form “tubes” when plated on Matrigel. B) Quantification shows that formation of linear structures (tubes or cords) as measured by counting branching points in the vascular plexus, is decreased by approximately 85%. C,D’-F’, D”-F”) Knockdown of Rasip1 inhibits endothelial cell migration. siRasip1-treated cells show slow healing rate in “scratch assay”. While untreated cells migrate quickly into the cell free area (D’,D”), siRasip1-treated cells migrate less than half the distance during the same time period (F’,F”). C) Quantification of endothelial migration in the scratch assay was determined by difference in relative diameter of scratch, before and after healing (D’-F’, compared to D”-F”), measured in μm on y axis (as shown).