Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: Mol Psychiatry. 2008 Jan 15;14(5):511–522. doi: 10.1038/sj.mp.4002148

Fig. 2 — 1-methyltryptophan abrogates depressive-like behavior in response to LPS without reducing expression of proinflammatory cytokines. Mice were implanted s.c. with a slow release pellet of the IDO competitive inhibitor, 1-MT or placebo. One week later, mice were injected i.p. with either non-pyrogenic saline or LPS (0.83 mg/kg). As in figure 1, the 24 h change in body weight (A) and the reduction in locomotor activity 6 or 24 h after LPS injection (B) were measured. The duration of immobility during the forced swim test was measured 24 h following administration of LPS (C). The duration of immobility during the tail suspension test was recorded 28 h post-LPS and measured either cumulatively (D) or in one min. increments over the 10 min. test (E). Immediately following behavioral testing, mice were sacrificed, perfused with ice-cold PBS and tissue collected. Steady-state expression of mRNA transcripts in the brain was measured by real-time RT-PCR for (F) TNF-α (G) IL-1β (H) IFN-γ and (I) IDO. Data represent means ± SEM (n=8 mice/group). Bars indicate statistical differences among groups. * P<0.05, ** P<0.01. Average Ct values for saline + LPS treated mice were, IFNγ=36 ± 1.7, TNF-α=27 ± 0.4, IL-1β=27 ± 0.3, and IDO=32 ± 1.6.

Fig. 2 — 1-methyltryptophan abrogates depressive-like behavior in response to LPS without reducing expression of proinflammatory cytokines. Mice were implanted s.c. with a slow release pellet of the IDO competitive inhibitor, 1-MT or placebo. One week later, mice were injected i.p. with either non-pyrogenic saline or LPS (0.83 mg/kg). As in figure 1, the 24 h change in body weight (A) and the reduction in locomotor activity 6 or 24 h after LPS injection (B) were measured. The duration of immobility during the forced swim test was measured 24 h following administration of LPS (C). The duration of immobility during the tail suspension test was recorded 28 h post-LPS and measured either cumulatively (D) or in one min. increments over the 10 min. test (E). Immediately following behavioral testing, mice were sacrificed, perfused with ice-cold PBS and tissue collected. Steady-state expression of mRNA transcripts in the brain was measured by real-time RT-PCR for (F) TNF-α (G) IL-1β (H) IFN-γ and (I) IDO. Data represent means ± SEM (n=8 mice/group). Bars indicate statistical differences among groups. * P<0.05, ** P<0.01. Average Ct values for saline + LPS treated mice were, IFNγ=36 ± 1.7, TNF-α=27 ± 0.4, IL-1β=27 ± 0.3, and IDO=32 ± 1.6.