Figure 8. BL-induced changes in the cell wall width of aquaporin-GFP expressing Arabidopsis cells.

(A) Confocal image of root tip tissue before (left) and 30 min after addition of 10 nM BL (right). Fluorescence intensity curves were recorded over the plasmalemmata-cell wall sections indicated by the white, alphabetically numbered lines and the FWHM values of their Gaussian fits calculated. (B–F) FWHM values of the plasmalemmata-cell wall sections indicated in A before (0 min) and 30 min after addition of 10 nM BL. (G) FWHM values of Calcofluor-stained anticlinal wall from hypocotyl cells recorded over the subcellular section indicated by the white line (8 µm) shown in the confocal image inlet at 0, 15 and 30 min after addition of 10 nM brassinolide. (H) FWHM values of the anticlinal wall autofluorescence from hypocotyl cells recorded over the subcellular section indicated by the white line (5 µm) shown in the confocal image inlet at 0 min and 30 min after addition of 10 nM BL. For the determination of the FWHM error in B to H see Material and Methods. For the statistical analysis of the aquaporin-GFP fluorescence measurements see Table S6.