Table 1. Effects of mitochondrial fusion and fission proteins on PINK1 phenotypes in different models.

Reference	System	Fission/Fusion manipulation	Effect	Interpretation
Poole et al [22]	Drosophila Genomic knockout	Drp1 LOF (heterozygote)	Lethality
		Drp1 expression or Opa1/Mfn2 LOF	Suppresses PINK1 LOF phenotypes	PINK1 promotes fission
Yang et al [23]	Drosophila Genomic knockout	Drp1 LOF (heterozygote)	Enhances PINK1 LOF phenotypes
		Drp1 expression or Opa1/Mfn2 LOF	Suppresses PINK1 LOF phenotypes
	COS7 (African green monkey) cells, transient siRNA	Fis1 expression or Drp1 expression	Suppresses PINK1 LOF phenotypes	PINK1 promotes fission
Deng et al. [36]	Drosophila Genomic knockout	Drp1 LOF (heterozygote)	Lethality
		Drp1 expression or Opa1/Mfn2 LOF	Suppresses PINK1 LOF phenotypes	PINK1 promotes fission/limits fusion
Dagda et al. [15]	SY5Y (Human) cells, stable siRNA	Drp1-dominant negative expression	Suppresses PINK1 phenotypes	PINK1 limits fission
This study	SY5Y (Human) cells, stable siRNA	Drp1 expression	Additive to PINK1 LOF phenotypes
		Drp1-knockdown, Opa1/Mfn2 expression	Suppresses PINK1 phenotypes	PINK1 limits fission or promotes fusion

Prior studies are listed where the effects of manipulating the expression or activity of fission and/or fusion proteins was measured against PINK1 loss of function mutations. LOF, loss of function.