Fig. 2.

Confocal images of multipotent neural precursor cell neurospheres. A) neurospheres were pulse labeled (2h) with BrdU, embedded in agarose, sectioned, and stained for BrdU. Positively stained, BrdU+ cells (green) were seen primarily at the periphery of the neurosphere; counterstaining was done with the nuclear dye 7-AAD. B) Neurospheres were differentiated for 5 days with serum, fixed in para-formaldehyde and processed for neuronal (β-tubulin, red) and astrocytic (GFAP, green) markers. C and D) Dissociated neurospheres were plated on matrigel and 2% serum for 2 days and stained with the astrocytic marker GFAP (green) or the immature neuronal markers doublecortin (red, C) or β-tubulin (red, D). E) Primary neurospheres were stained for CD133 (green); counterstaining was done with the nuclear dye 7-AAD.