Fig. 5.

Neurogenesis in the dentate subgranular zone is reduced 2 months after local brain irradiation. Newly born neurons were co-labeled with antibodies against BrdU and the neuron specific antigen NeuN and the fractions of double labeled cells were quantified with confocal microscopy. Approximately 85% of newly born cells differentiated into neurons in sham-irradiated controls and there was significant (p < 0.001) dose-related decrease after irradiation. Each datum point represents 4 mice and error bars are SEM. Adapted from Mizumatsu et al (22) with permission.