Figure 3.
Partial complementation of the UV sensitivity phenotype of a Δlon strain by lon mutants. The graph represents the efficiency of plating after UV exposure as a function of arabinose concentration. Strains were grown in LB medium overnight at 32°C. After serial dilution, cells were spotted on LB plates containing arabinose at the final concentration indicated in the graph. Plates were UV irradiated (15–20 J/m2). After overnight incubation at 32°C, colonies were counted and compared with the titer without UV irradiation. Strains were SG22542 (lon+)/pBAD24 (▴), SG22569 (Δlon) containing the pBAD24 vector (●), pBADlon+ (■), pBADlonK362Q (□), pBADlonS679A (○), or pBADlonK362Q-S679A (▵)