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. 1999 May 25;96(11):6064–6071. doi: 10.1073/pnas.96.11.6064

Figure 5.

Figure 5

SulA solubility. Differential centrifugations were performed as described in Materials and Methods. Samples of LVM781 (Δlon) containing pBADlon+ or pBADlonS679A were probed with anti-SulA antibody. Cells: Total extract prepared by TCA precipitation. Lysate: Total extract after French press treatment. Low-speed pellet: Resuspended pellet from centrifugation for 6 min at 3,500 rpm. High-speed pellet: Resuspended pellet from centrifugation for 15 min at 14,000 rpm. High-speed supernatant: From centrifugation for 15 min at 14,000 rpm.