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. Author manuscript; available in PMC: 2010 Apr 10.
Published in final edited form as: Mol Cell Endocrinol. 2009 Jan 20;302(1):12–17. doi: 10.1016/j.mce.2008.12.013

Figure 3.

Figure 3

Time course of miRNA induction by GnRH in LβT2 gonadotropes. Cells were treated with either 100 nM GnRH or vehicle and total RNA samples were isolated after 0, 1, 3, 6 and 24 hours of treatment. Expression levels of let-7a, miR-132 and miR-212 were determined by hairpin TaqMan real time PCR assays. Both miR-132 and miR-212 have similar trajectories. Increased levels were observed after 1 hour of GnRH treatment. Induction peaked at 6 hours and the miR-132 and miR-212 levels remained elevated after 24 hours. No significant induction was found in let-7a. This experiment is representative of three independent studies. Error bars denote standard error of the mean of three replicate samples.