Fig. 7.
AP18 is a potent, selective and reversible antagonist for TRPA1. (a, b) AP18 inhibits increases in intracellular calcium evoked by cinnamaldehyde (Cinn; a; FLIPR technique) and iodoacetamide (IA; b; Fura-2 ratiometric calcium imaging) in CHO cells expressing TRPA1. (c) AP18 (20 μM) strongly inhibited the Cinn-activated TRPA1 currents in inside-out macropatches derived from Xenopus oocytes. The selectivity profile of AP18 is described in the box inset.