TABLE 3.

CYP2D6 holoprotein levels (percentage of total protein) in the purified recombinant CYP2D6 enzymes reported in the literature

Average molecular mass of each protein was calculated according to its corresponding amino acid sequence described in that article unless otherwise indicated.

Protein	Molecular Mass	Theoretical CYP2D6 Content	Experimental CYP2D6 Content	Holoprotein Level	Expression System	Reference
	Da	mg/ml	mg/ml	%
CYP2D6m-Δ 21	53,262.2	18.8	10.0	53.2	Escherichia coli	Gillam et al., 1995
[His]6-CYP2D6-Δ 25	55,535.7	18.0	7.12a, 2.67b	39.6a, 14.8b	E. coli	Kempt et al., 1995
CYP2D6-Δ	48,500c	20.6	12.2	59.2	Yeast	Imaoka et al., 1996
CYP2D6-Ext	56,235.1	17.8d, 20.0c	18.3	91.5d	Baculovirus (insect cell)	Modi et al., 1996
CYP2D6.1	55,769.5	17.9	11.5	64.2	Baculovirus (insect cell)	Yu et al., 2002
CYP2D6m	55,647.3	18.0	12.5	69.4	E. coli	Ng et al., 2003
CYP2D6m-Δ-[His]4	53,705.7	18.6	11.0	59.1	E. coli	Roland et al., 2006

^aPurified without detergent.

^bPurified with detergent.

^cValues reported in corresponding papers.

^dValue we calculated according to its molecular mass.