Fig. 2.

Human DC mannose receptors allow for increased binding of insect cell-derived Igs. (A) Human DCs were incubated at 4 °C with FITC-labeled KLH, human IgG-KLH, or recombinant BV-derived human IgG-KLH. IgGs were conjugated to KLH via glutaraldehyde and evaluated for surface binding. (B) Murine A20 Id produced in both BV or Hyb systems were labeled with Alexa 647 and incubated for 2 h at 37 °C with human DCs to determine surface binding of the Igs. (C) BV A20 Id-Alexa 647, Hyb A20 Id-Alexa 647, or FITC-dextran were incubated with human DCs in the presence of various inhibitors including mannan, control IgG antibody, anti-MR blocking antibody, or cytochalasin D, and bound antigen quantitated by flow cytometry.