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Vaccination with BV Id-KLH maleimide conjugate results in higher tumor antigen-specific antibody titers than glutaraldehyde conjugates. Groups of four mice were vaccinated with BV or Hyb Id conjugated to KLH via either maleimide or glutaraldehyde weekly for 3 weeks together with GM-CSF. Mice were bled 10 days later and anti-Id antibody titers determined by ELISA. Error bars represent standard deviations among serum values from individual mice.
