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. 2009 Feb 26;28(8):1016–1028. doi: 10.1038/emboj.2009.47

Figure 4.

Figure 4

AKAP450 is required for MT nucleation at the GA. (A–D) Control (A, C) or AKAP450-depleted (B, D) cold-treated cells after 1 min of rewarming (A, B) or NZ-treated cells after 5 min washout (C, D) were labelled for AKAP450 (red), tubulin (green) and GMAP210 (blue). A1–D1 are inverted images of tubulin staining. A2–D2 are merged images. In E, F, AKAP450 nondepleted (E) or AKAP450-depleted (F) cold-treated cells after 5 min at room temperature are shown labelled for AKAP450 in red, tubulin in green and GMAP210 in blue. E1 and F1 are inverted images of tubulin staining. Merged images are shown in E2 and F2. Arrows indicate the centrosome. Bars, 10 μm.