Figure 6.

The P261S and D388V missense Arr2 proteins are not phosphorylated in vitro and have defects in releasing from rhodopsin. (A, Left) Autoradiograph of γ-³²P-labeled proteins isolated from Drosophila head lysates in the presence of 0.5 mM Ca²⁺; (A, Right) The same gel transferred to nitrocellulose and probed with antibodies specific for Arr2. P261S and D388V represent missense mutations in Arr2. w, white-eyed control flies. (B) Western blot of fractionated P261S and D388V mutant heads that were exposed to 10 s of blue light (B) or 10 s of blue light followed by 60 s of orange light (BO). Pellet (P) and supernatant (S) fractions were subjected to SDS/PAGE and Western analysis with antibodies directed against Arr2 and Rh1.