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. 2009 Mar 12;28(8):1111–1120. doi: 10.1038/emboj.2009.49

Figure 5.

Figure 5

XPF- and XPG-dependent colocalization of PCNA and CAF-1 with XPC. XPCS1RO (XP-G) cells, untransduced or transduced with XPG-WT or XPG-E791A (A, C) and XP2YO (XP-F) cells, untransduced or transduced with XPF-WT or XPF-D676A (B, D), were grown on coverslips and locally irradiated with a UV dose of 150 J/m2 through filters with 5 μm pores and fixed 0.5 h after irradiation. The cells were immunolabelled with antibodies against XPC (green), PCNA (red) and CHAF150, the largest subunit of CAF-1 (red). Merged images indicate the overlay of XPC, PCNA or CHAF150 and DAPI staining. Scale bars, 10 μm.