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. 2009 Mar 12;28(8):1142–1156. doi: 10.1038/emboj.2009.60

Figure 1.

Figure 1

Mre11 is required for normal replication fork progression in the presence of HU. Exponentially growing wild-type (JC604) and mre11Δ (JC616) cells were labelled for 3 h with 200 μg/ml BrdU in the presence of 200 mM HU. Genomic DNA fibres were stretched on silanized coverslips by DNA combing and newly replicated DNA was detected with an anti-BrdU antibody. DNA fibres were counterstained with an anti-ssDNA antibody. (A) Representative fibres of wild type (top panel) and mre11Δ (bottom panel) are shown with DNA in red and BrdU in green; Bar: 50 kb. (B) Distribution of BrdU tracks length in HU-treated cells. Box: 25–75 percentile range. Whiskers: 10–90 percentile range. Median (vertical bars) and mean values (italics) are indicated in kb. ***P<0.0001; Mann–Whitney rank sum test. (C) Distribution of BrdU tracks length in untreated wt and mre11Δ cells. ns: P=0.62; Mann–Whitney rank sum test.