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. 2009 Mar 12;28(8):1142–1156. doi: 10.1038/emboj.2009.60

Figure 2.

Figure 2

Stability of replisome components in mre11Δ cells. (A) Genomic regions amplified for ChIP analysis on Chr VI are as described earlier (Cobb et al, 2003, 2005) and correspond to early firing origin ARS607 (filled symbol) and a nonorigin site, +14 kb (open symbols). ChIP was performed using the following strains with either αMyc (9E10) or αHA (F-7, Santa-Cruz) antibodies on cells released from α-factor into YPAD+0.2 M HU (B) for HA-Polα in wild-type (JC539) and mre11Δ (JC538) cells; (C) Myc-Polɛ was monitored after released into 0.2 M HU comparing wild-type (JC285) with mre11Δ (JC388) and (D) rad50Δ (JC796) and xrs2Δ (JC757) cells. (E) Myc-Polɛ in wild-type (JC285) and mre11Δ (JC388) cells was monitored after released into YPAD without HU at 19°C. (F) Myc-Mcm4 was monitored after released into 0.2 M HU comparing wild type (JC171) with mre11Δ (JC559). (G) HA-Ddc2 in wild-type (JC257) and mre11Δ (JC549) cells was determined after release into 0.2 M HU. Error bars represent the standard deviation in fold enrichment of multiple runs of at least three different ChIP experiments.