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. 2009 Mar 12;28(8):1142–1156. doi: 10.1038/emboj.2009.60

Figure 4.

Figure 4

The MRX complex functions at the fork independently of Mre11 nuclease activity. ChIP using αMyc (9E10) and αHA (F-7, Santa-Cruz) antibodies as described in Figure 2 was performed on (A) on HA-Rad50 and Myc-Orc2 in wild-type (J764) and (B) mre11Δ (JC743) cells, where enrichment at ARS607 (solid line) and +14 kb from ARS607 (speckled line) is monitored at the indicated time points after release into 0.2 M HU. (C) Drop assays on exponentially growing cells of 1:10 serial dilutions on SC-TRP plates +10 mM HU at 30°C or (D) ChIP on Myc-Polɛ using αMyc (9E10) as described in Figure 2 were performed with wild-type (JC285) cells after transformation with pRS314 vector alone (WT +pRS314, ▪), and in mre11Δ (JC388) cells after transformation with pRS314 vector alone (mre11Δ+pRS314, ▴), or pRS314 carrying either full length MRE11 (mre11Δ+pMRE11, ▵) or mre11-3 (mre11Δ+pmre11-3, ○) and mre11-2 (mre11Δ+pmre11-2, •) constructs from cultures grown in SD-TRP media. (E) ChIP using αMyc (9E10) was performed on Myc-Polɛ in wild-type (JC285) and sae2Δ (JC686) cells as described in Figure 2.