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. 2009 Mar 12;28(8):1142–1156. doi: 10.1038/emboj.2009.60

Figure 5.

Figure 5

Mre11 shows synergistic interactions with replisome components. (A) Drop assays on YPAD±10 mM HU were performed with exponentially growing cultures at permissive temperature (25°C) with 1:5 serial dilutions before 2 days incubation at 30°C (B) Cell viability was monitored as colony outgrowth from asynchronous cultures grown at permissive temperature (25°C) before transient exposure to 0.2 M HU at 30°C for the indicated times with values normalized to survival at time point 0 and (C) the phosphorylation status of Rad53 was monitored by western blot analysis after cells were blocked in G1 with α-factor and released in 0.2 M HU for the indicated time at 30°C using anti-Rad53 antibodies (yC-19, Santa Cruz) for the following strains: wild type (JC467), mre11Δ (JC371) mcm10-43 (JC216), mcm10-43/mre11Δ (JC554), pol2-12 (JC617), pol2-12/mre11Δ (JC553), mcm5-3 (JC227), mcm5-3/mre11Δ (JC564), pol2-16 (JC583) pol2-16/mre11Δ (JC619), tel1Δ (JC65), mcm10-43/tel1Δ (JC591), pol2-12/mre11Δ (JC612).