Figure 6.
Epistatic analysis between replisome alleles with mre11Δ and rad9Δ or rad51Δ. (A–D) Cell viability was monitored as colony outgrowth from asynchronous cultures grown at permissive temperature (25°C) before transient exposure to 0.2 M HU at 30°C for the indicated times with values normalized to survival at time point 0. All experiments were performed at the same time as Figure 5B for the following strains: rad9Δ (JC176), rad9Δ/mre11Δ (JC568) mcm10-43/rad9Δ (JC569), mcm10-43/rad9Δ/mre11Δ (JC570), pol2-12/rad9Δ (JC618), pol2-12/rad9Δ/mre11Δ (JC572), rad51Δ (JC445), rad51Δ/mre11Δ (JC633) mcm10-43/rad51Δ (JC634), mcm10-43/rad51Δ/mre11Δ (JC629), pol2-12/rad51Δ (JC630), pol2-12/rad51Δ/mre11Δ (JC683).
