Table 1.
Kinetic Parameters for GAOX at 10 °C, pH 7.0 with Protio- and Deuterio- Substrates.
Parameter | H2-OMeGal | D2-OMeGal |
---|---|---|
kcat, s−1 | 1165 (5) | 49 (2) |
kcat/Km(OMeGal), M−1, s−1 | 8.3 (0.6) × 103 | 2.6 (0.4) × 102 |
kcat/Km(O2), M−1, s−1 | 2.6 (0.3) × 106 | 1.5 (0.2) × 106 |
Km(OMeGal), mM | 1.4 (0.1) × 102 | 1.9 (0.2) × 102 |
Km(O2), μM | 4.4 (0.3) × 102 | 77 (10)a |
Value was calculated from kcat and kcat/Km(O2).