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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2008 Oct 19;41(5):1148–1156. doi: 10.1016/j.biocel.2008.10.013

Figure 4.

Figure 4

Auto-activation of Ov-APR-1 from a pro-enzyme to its mature form at acidic pH. Recombinant Ov-APR-1 (pro-enzyme) was incubated in 50 mM sodium acetate at half-unit pH increments from pH 2–5 at 37°C for 18 h. C – control Ov-APR-1 protein in PBS, pH 7.2; 2.0 – 5.0 - pH values at which Ov-APR-1 was incubated to observe auto-activation from pro- (upper band) to mature form (lower band).