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. Author manuscript; available in PMC: 2009 May 18.
Published in final edited form as: Clin Immunol. 2007 Aug 9;125(1):5–15. doi: 10.1016/j.clim.2007.07.002

Figure 3. Co-localization of FcRγ-chain with the CD3ε subunit of the TCR/CD3 complex in effector CD4 T cells.

Figure 3

Resting CD4 T cells and differentially activated CD4 T cells were directly fixed (uncapped) or activated with IgM-anti-CD3ε Ab at 37°C to induce CD3ε cap formation before fixation (CD3–capped). Cells were surface stained with anti-CD3ε-FITC (green) and intracellularly stained with anti-FcRγ and its TRITC-conjugated secondary anti-rabbit antibody (red). The merged images (yellow) indicate co-localization of CD3ε with FcRγ. Controls with secondary Ab alone did not show red or green fluorescence (data not shown). The magnification is 100X. Results are representative of three experiments.