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. Author manuscript; available in PMC: 2009 May 18.
Published in final edited form as: Biofactors. 2008;32(1-4):13–22. doi: 10.1002/biof.5520320103

Table 1.

Comparison between Q1 and DBQ.

Q1 DBQ
(A) Original complex I suspension.
 (a) Rate of NADH oxidation (μmoles mg−1 min−1) 5.0 7.9
 (b) Inhibition (%) by piericidin A 98.5 99.0
(B) Proteoliposomes suspension.
 (a) Rate of NADH oxidation 0.32 0.43
 (b) Rate after CCCP addition 0.83 2.4
 (c) Respiratory control ratio 2.6 5.7
 (d) Inhibition (%) by piericidin A 88.5 99.0

Experimental conditions: The buffer solution contains 50 μM NADH, 100 μM Q, or DBQ, 15 mM Na2SO4 and 5 mM HEPES (pH. 7.5), 30°C. (A) 3 μg/ml Complex I, 0.1 mg/ml asolectin and 0.1 % dodecyl maltoside were added to the buffer. (B) Reconstituted proteoliposomes (protein concentration: 1.4 mg/ml; phospholipids: 70 mg/ml) was suspended in the same buffer solution as described before.