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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: Biochemistry. 2009 Feb 10;48(5):1077–1083. doi: 10.1021/bi801871h

Figure 3. αSyn effects on yeast growth are not rescued by overexpression of PLD.

Figure 3

(A) Yeast cells with a temperature-sensitive mutation in SEC14 (sec14-1) grow normally at 23°C, but are unable to grow at 37°C, unless they acquire various “bypass mutations” (sec14-1, vector, 37°C). As reported previously (24), αSyn expression prevents the ability of the sec14-1 bypass mutants to grow at the restrictive temperature (sec14-1, αSyn, 37°C). (B) Deletion of the choline kinase 1 gene (cki1Δ), allows the sec14-1 mutants to grow at the restrictive temperature in a PLD-dependent manner, and αSyn expression in these cells blocks this rescue effect. (C) Deletion of SPO14 (spo14Δ) in the sec14-1/cki1Δ mutants abolishes their ability to grow at the restrictive temperature. Overexpression of functional SPO14 restores growth to sec14-/cki1Δ/spo14Δ cells (C) but is unable to rescue the αSyn-induced growth defect (D). (n=3)