BK β4 subunits in HNS nerve terminals. Single HNS nerve terminals
magnified at 63× immunolabeled with: A, anti-β1-conjugated and
mouse secondary Alexa 594-conjugated antibody; or B, anti-β4- and mouse
secondary Alexa 594-conjugated antibody. Nerve terminals are counterstained
with: 1, anti-AVP neurophysin- and rabbit secondary Alexa 350-conjugated
antibody; and 2, anti-OXT-conjugated and rabbit secondary Alexa 488-conjugated
antibody. C, pie charts of the optical density of β1 (left chart) and
β4 (right chart) immunofluorescence in the dendrite (red), soma (blue),
and terminal (yellow) compartments of SON neurons.