Figure 3. Distribution of BT-15d-PGJ₂ between cellular and media protein adducts.

Cells were incubated with BT-15d-PGJ₂ (10 μM) in 0% or 0.5% serum containing media for 4h. Media were then collected and Triton soluble cell lysates and SDS soluble cell pellets prepared for analysis of BT-15d-PGJ₂ protein adducts in the cell by SDS-PAGE followed by western blotting. Panel A: BT-15d-PGJ₂ adduct formation in cell fractions in the absence and presence of FBS determined by western blot and protein stained gel from media. Protein bands showing decreased adduct formation after incubation with 0.5% FBS are indicated with arrows. Panel B: BT-15d-PGJ₂ adduct formation in media and protein staining in the absence and presence of 0.5% FBS. The quantitation of these data is reported in Table 1.