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. Author manuscript; available in PMC: 2009 May 18.
Published in final edited form as: Neuroscience. 2007 Apr 3;146(3):1193–1211. doi: 10.1016/j.neuroscience.2007.01.072

Fig. 6.

Fig. 6

Validation of the P469 polyclonal antibody. (a) Western blot analysis of untagged (58 kDa) and YFP-tagged (83 kDa) mPMAT or hPMAT expressed in MDCK cells. Untransfected or YFP-transfected cells were used as controls. (b). Immunocytochemical analysis of hPMAT (stable transfection) and mPMAT (transient transfection) in MDCK cells. Cells were stained with the P469 anti-PMAT antibody (1:200) and the nuclei were counterstained with Topro-3 dye.