Stimulation of 45Ca2+ uptake by ANP, SNP, and 8-Br-cGMP in freshly isolated CNT and CCD tubules. Freshly immunodissected CNT and CCD tubules were stimulated with ANP (100 nM), SNP (100 μM), and 8-Br-cGMP (100 μM) in the presence of tracer amount 45Ca2+ for 10 min at 37°C. Basal 45Ca2+ uptake is set at 100%, to which all values are related. Values are means ± SEM (n = 5). ∗, P < 0.05, significantly different from basal 45Ca2+ uptake.