Figure 5.

cGMP-stimulated Ca²⁺ reabsorption in CNT/CCD monolayers expressing cGK proteins of different membrane-association properties. Monolayers were noninfected or infected with adenovirus for expressing either cGK II, a myristoylation-deficient cGK II mutant (G2A), cGK Iβ, or a chimer of the N terminus of cGK II fused to the N terminus of full-length cGK Iβ. Two days after infection, monolayers either were not stimulated (open bars) or stimulated with 100 nM basolateral ANP (shaded bars) or 100 μM 8-Br-cGMP added to both sides (solid bars). Values are means ± SEM of three experiments. ∗, P < 0.05, significantly different from control values.