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Transcellular Na+ transport is not affected by cGK expression. Monolayers of CNT/CCD cells either were noninfected or infected with adenovirus for expressing cGK Iβ or cGK II. Two days after infection, the effect of 100 μM 8-Br-cGMP (both sides, for the period indicated in the figure) on the ISC was determined. Subsequently, transcellular Na+ transport was blocked by addition of 10 μM benzamil to the apical compartment. Representative traces of three experiments are shown.
