Fig. 6.

SINV virulence is affected by mutations in the nsP3 macro domain in mature neurons. The effect of mutations on SINV virulence in undifferentiated (A) and 3 week-differentiated (B) CSM14.1 cells. Cells were infected with SINV WT, SINV SM10, SINV SM24, or SINV DM (MOI = 10), or mock infected. Live cells were counted at indicated days post-infection by trypan blue exclusion assay, and percentage of D0 cells was calculated. Representative data from 3 independent experiments are presented. In each experiment, triplicate samples were assessed. Error bars indicate SEM. ⁎P < 0.05; ⁎⁎P < 0.01; ⁎⁎⁎P < 0.001; Student's t-test comparing SINV WT and mutants.