Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Gene. 2009 Mar 26;440(1-2):9–15. doi: 10.1016/j.gene.2009.03.011

Figure 1. DNA vectors expressing either DnaK or GroEL and their cognate co-chaperone partners.

Figure 1

(A) Plasmid maps of DNA constructs expressing DnaK (pXCK-K), GroEL (pXCK-EL), DnaJ and GrpE (pXCK-E/J) and GroES (pXCK-ES). DnaK and GroEL were cloned into identical plasmid vectors to minimize any possible effects from expression in different backgrounds. (B) Schematic diagram of the heat shock fusion protein showing the position of the thrombin cleavage site and 6His tag. (C) DNA sequence of the multiple-cloning restriction sites between the sequence encoding the thrombin cleavage site and the 6His tag. (D) BL21[DE3] cells were transformed with the indicated plasmids and protein expression was induced with IPTG. Cells were suspended in SDS-lysis buffer and proteins were analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue.