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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Gene. 2009 Mar 26;440(1-2):9–15. doi: 10.1016/j.gene.2009.03.011

Figure 2. PrP partitions in the soluble fraction when expressed as a chaperone fusion.

Figure 2

BL21[DE3] cells were transformed with the indicated plasmids and protein expression was induced with 1mM IPTG. Cell lysates were prepared and proteins were fractionated, analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue. (A) Total cell extract, (B) Soluble fraction and (C) Inclusion bodies.