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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Gene. 2009 Mar 26;440(1-2):9–15. doi: 10.1016/j.gene.2009.03.011

Figure 4. (A) The DnaK-PrP is efficiently cleaved by thrombin.

Figure 4

DnaK and DnaK-PrP were affinity purified using Ni-NTA chromatography. DnaK-PrP was incubated with thrombin for the indicated times. The proteins were analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue. (B) DnaK has enzymatic activity when fused to PrP. DnaK and DnaK-PrP were affinity purified by Ni-NTA chromatography. Equal amounts of each protein were used in autophosphorylation reactions for the indicated times. The products of the reactions were analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue (top panel). The dried gel was exposed to a phosphorimager cassette to assess 32P incorporation (bottom panel).